Anti-MC1-R antibody [EPR6530] (ab125031)
![Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139174-anti-mc1-r-antibody-epr6530-western-blot.jpg "Anti-MC1-R antibody [EPR6530] (ab125031)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6530] to MC1-R
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MC1-R antibody [EPR6530]
See all MC1-R primary antibodies -
Description
Rabbit monoclonal [EPR6530] to MC1-R -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human MC1-R. The exact sequence is proprietary.
(Peptide available asab195973) -
Positive control
- WB: A375 and Human melanoma lysates IHC-P: Human melanoma tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as MC1 Receptor.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.93 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6530 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MC1-R antibody [EPR6530] (ab125031)All lanes : Anti-MC1-R antibody [EPR6530] (ab125031) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Human melanoma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 35 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139173-anti-mc1-r-antibody-epr6530-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody [EPR6530] (ab125031)ab125031, at a 1/500 dilution, staining MC1-R in paraffin embedded Human melanoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-139172-anti-mc1-r-antibody-epr6530.jpg)
OI-RD Scanning - Anti-MC1-R antibody [EPR6530] (ab125031) -
![Anti-MC1-R antibody [EPR6530] (ab125031)](https://www.abcam.com/ps/products/125/ab125031/Images/ab125031-4-benefits-of-recombinant-antibodies.png)
Anti-MC1-R antibody [EPR6530] (ab125031)
