Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MBD1 antibody [EPR3564] (ab108510)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3564] to MBD1
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MBD1 antibody [EPR3564]
    See all MBD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3564] to MBD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, A431, 293T and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. ICC: MCF7 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR3564

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)
    Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)
    All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : MBD1 knockout HAP1 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 40 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 67 kDa
    Observed band size: 70-80 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab108510 observed at 70-80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab108510 was shown to react with MBD1 in wild-type HAP1 cells in western blot with loss of signal observed in MBD1 knockout sample. Wild-type and MBD1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab108510 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-MBD1 antibody [EPR3564] (ab108510)
    Immunocytochemistry/ Immunofluorescence - Anti-MBD1 antibody [EPR3564] (ab108510)
    ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD1 antibody [EPR3564] (ab108510)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD1 antibody [EPR3564] (ab108510)

    ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)
    Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)
    All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 67 kDa

  • Anti-MBD1 antibody [EPR3564] (ab108510)
    Anti-MBD1 antibody [EPR3564] (ab108510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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