Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3564] to MBD1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-MBD1 antibody [EPR3564]
See all MBD1 primary antibodies
Description

Rabbit monoclonal [EPR3564] to MBD1
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, A431, 293T and Jurkat whole cell lysate (ab7899). IHC-P: Human tonsil tissue. ICC: MCF7 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3564
Isotype

IgG
Research areas

Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)

All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : MBD1 knockout HAP1 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 40 µg per lane.

Performed under reducing conditions.

Predicted band size: 67 kDa
Observed band size: 70-80 kDa
why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab108510 observed at 70-80 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108510 was shown to react with MBD1 in wild-type HAP1 cells in western blot with loss of signal observed in MBD1 knockout sample. Wild-type and MBD1 knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108510 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-MBD1 antibody [EPR3564] (ab108510)

ab108510, at 1/100 dilution, staining MBD1 in MCF7 cells by Immunofluorescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MBD1 antibody [EPR3564] (ab108510)

ab108510, at 1/100 dilution, staining MBD1 in paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-MBD1 antibody [EPR3564] (ab108510)

All lanes : Anti-MBD1 antibody [EPR3564] (ab108510) at 1/500 dilution

Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 67 kDa
Anti-MBD1 antibody [EPR3564] (ab108510)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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