Anti-MAVS antibody (ab31334)
Key features and details
- Rabbit polyclonal to MAVS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAVS antibody
See all MAVS primary antibodies -
Description
Rabbit polyclonal to MAVS -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human MAVS aa 100-200.
(Peptide available asab25852)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab31334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 57, 75 kDa (predicted molecular weight: 57 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57, 75 kDa (predicted molecular weight: 57 kDa).Application notesIs unsuitable for ICC/IF.Target
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Function
Required for innate immune defense against viruses. Acts downstream of DDX58 and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. -
Tissue specificity
Present in T-cells, monocytes, epithelial cells and hepatocytes (at protein level). Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. -
Sequence similarities
Contains 1 CARD domain. -
Domain
Both CARD and transmembrane domains are essential for antiviral function. The CARD domain is responsible for interaction with DDX58 and IFIH1. -
Post-translational
modificationsUbiquitinated; undergoes 'Lys-48'-linked polyubiquitination catalyzed by ITCH; ITCH-dependent polyubiquitination is mediated by the interaction with PCBP2 and leads to MAVS proteasomal degradation. -
Cellular localization
Mitochondrion outer membrane. - Information by UniProt
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Database links
- Entrez Gene: 57506 Human
- Entrez Gene: 228607 Mouse
- Omim: 609676 Human
- SwissProt: Q7Z434 Human
- SwissProt: Q8VCF0 Mouse
- Unigene: 570362 Human
- Unigene: 287226 Mouse
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Alternative names
- CARD adapter inducing interferon beta antibody
- CARD adaptor inducing IFN beta antibody
- Cardif antibody
see all
Images
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Western blot - Anti-MAVS antibody - ChIP Grade (ab31334)All lanes : Anti-MAVS antibody (ab31334) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57,75 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible non-specific binding)The bands at 75 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763. The band at 51 kDa may represent non-specific antibody binding.
Protocols
Datasheets and documents
References (13)
ab31334 has been referenced in 13 publications.
- Hyun J et al. HIV and HCV augments inflammatory responses through increased TREM-1 expression and signaling in Kupffer and Myeloid cells. PLoS Pathog 15:e1007883 (2019). PubMed: 31260499
- Song N et al. MAVS O-GlcNAcylation Is Essential for Host Antiviral Immunity against Lethal RNA Viruses. Cell Rep 28:2386-2396.e5 (2019). PubMed: 31461653
- Zhang M et al. Low level expression of the Mitochondrial Antiviral Signaling protein (MAVS) associated with long-term nonprogression in SIV-infected rhesus macaques. Virol J 15:159 (2018). PubMed: 30326919
- MacDuff DA et al. HOIL1 Is Essential for the Induction of Type I and III Interferons by MDA5 and Regulates Persistent Murine Norovirus Infection. J Virol 92:N/A (2018). PubMed: 30209176
- Loetsch C et al. Cytosolic Recognition of RNA Drives the Immune Response to Heterologous Erythrocytes. Cell Rep 21:1624-1638 (2017). WB ; Mouse . PubMed: 29117566
Images
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Western blot - Anti-MAVS antibody - ChIP Grade (ab31334)All lanes : Anti-MAVS antibody (ab31334) at 1 µg/ml
Lane 1 : Hela Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 57 kDa
Observed band size: 57,75 kDa why is the actual band size different from the predicted?
Additional bands at: 51 kDa (possible non-specific binding)The bands at 75 and 57 kDa correspond to the bands seen in a paper by Seth et al., 2005 (See Supplementary Information). PubMed: 16125763. The band at 51 kDa may represent non-specific antibody binding.
