Anti-MATE-1 antibody (ab92295)
Key features and details
- Goat polyclonal to MATE-1
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MATE-1 antibody
See all MATE-1 primary antibodies -
Description
Goat polyclonal to MATE-1 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human MATE-1 aa 528-541 (internal sequence) (Cysteine residue).
Sequence:C-PEHPQDGAKLSRKQ
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Positive control
- WB: Human liver tissue lysate; ICC: HepG2 cells.
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General notes
Protein previously labeled as SLC47A1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-MATE-1 antibody (ab92295)
Immunocytochemistry analysis of paraformaldehyde fixed HepG2 cells labeling MATE-1. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Western blot - Anti-MATE-1 antibody (ab92295)All lanes : Anti-MATE-1 antibody (ab92295) at 0.1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human liver lysate (in RIPA buffer) with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size: 62 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
