Anti-Malaria ELISA Kit (ab178649)
Key features and details
- Sensitivity: 95.9 %
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Anti-Malaria ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Positive 22 2.8% Positive 22 3.9% Negative 22 5.5% Inter-assay Sample n Mean SD CV% Positive 24 3.2% Positive 24 4.8% Negative 24 10.3% -
Sample type
Serum, Hep Plasma, Cit plasma -
Assay type
Indirect -
Sensitivity
= 95.9 % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s anti-Malaria Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma.
A 96-well plate has been precoated with Malaria antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG and anti-Human IgM conjugate is added to the wells, which binds to the immobilized Malaria antigens. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Malaria IgG or Malaria IgM sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover Foil 1 unit IgG Sample Diluent 1 x 100ml Malaria Anti-IgG and Anti-IgM HRP Conjugate 1 x 20ml Malaria Coated Microplate (12 x 8 wells) 1 unit Malaria Cut-off Control 1 x 3ml Malaria Negative Control 1 x 2ml Malaria Positive Control 1 x 2ml Stop Solution red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml -
Research areas
Images
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Serologic ELISA assay principleSpecific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
