Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LysRS antibody [EPR7921] (ab129080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7921] to LysRS
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-LysRS antibody [EPR7921]
    See all LysRS primary antibodies

  • Description

    Rabbit monoclonal [EPR7921] to LysRS

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human LysRS aa 550-650.
    Database link: Q15046

  • Positive control

    • Jurkat, HeLa, 293T, HepG2, and Caco 2 cell lysates; Human colon tissue This antibody gave a positive result when used in the following methanol fixed cell lines: CACO-2

  • General notes

     

     

    Previously labelled as Lysyl tRNA synthetase. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-LysRS antibody [EPR7921] (ab129080)
    Western blot - Anti-LysRS antibody [EPR7921] (ab129080)
    All lanes : Anti-LysRS antibody [EPR7921] (ab129080) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : HepG2 cell lysate
    Lane 5 : Caco 2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 68 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] (ab129080)
    Immunocytochemistry/ Immunofluorescence - Anti-LysRS antibody [EPR7921] (ab129080)

    ICC/IF image of ab129080 stained CACO-2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab129080 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] (ab129080)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LysRS antibody [EPR7921] (ab129080)

    ab129080, at a dilution of 1/50, staining LysRS in paraffin embedded Human colon tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-LysRS antibody [EPR7921] (ab129080)
    OI-RD Scanning - Anti-LysRS antibody [EPR7921] (ab129080)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LysRS antibody [EPR7921] (ab129080)
    Anti-LysRS antibody [EPR7921] (ab129080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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