Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8188(2)] to LRRC15
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-LRRC15 antibody [EPR8188(2)]
    See all LRRC15 primary antibodies

  • Description

    Rabbit monoclonal [EPR8188(2)] to LRRC15

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LRRC15 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y and fetal brain lysates; SH-SY5Y cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution

    Lanes 1 & 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 2 : Human brain lysates
    Lane 3 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling LRRC15 with purified ab150376 at 1:100 dilution (9.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

    Immunofluorescent analysis of SH-SY5Y cells labeling LRRC15 with ab150376 (unpurified) at a 1/50 dilution.

  • Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution (unpurified)

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : fetal brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

    Predicted band size: 64 kDa

  • Anti-LRRC15 antibody [EPR8188(2)] (ab150376)
    Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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