Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8188(2)] to LRRC15
Suitable for: WB, ICC/IF
Reacts with: Rat, Human

Product name

Anti-LRRC15 antibody [EPR8188(2)]
See all LRRC15 primary antibodies
Description

Rabbit monoclonal [EPR8188(2)] to LRRC15
Host species

Rabbit
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human LRRC15 aa 50-150. The exact sequence is proprietary.
Positive control
- SH-SY5Y and fetal brain lysates; SH-SY5Y cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8188(2)
Isotype

IgG
Research areas

Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution

Lanes 1 & 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 2 : Human brain lysates
Lane 3 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 64 kDa
Observed band size: 64 kDa
Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Immunocytochemistry/ Immunofluorescence analysis of U-87 MG (Human glioblastoma-astrocytoma epithelial cell) cells labeling LRRC15 with purified ab150376 at 1:100 dilution (9.6 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunocytochemistry/ Immunofluorescence - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Immunofluorescent analysis of SH-SY5Y cells labeling LRRC15 with ab150376 (unpurified) at a 1/50 dilution.
Western blot - Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

All lanes : Anti-LRRC15 antibody [EPR8188(2)] (ab150376) at 1/1000 dilution (unpurified)

Lane 1 : SH-SY5Y cell lysate
Lane 2 : fetal brain cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit antibody at 1/2000 dilution

Predicted band size: 64 kDa
Anti-LRRC15 antibody [EPR8188(2)] (ab150376)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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