Anti-LINE-1 ORF2p antibody (ab106004)
Key features and details
- Chicken polyclonal to LINE-1 ORF2p
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Overview
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Product name
Anti-LINE-1 ORF2p antibody -
Description
Chicken polyclonal to LINE-1 ORF2p -
Host species
Chicken -
Specificity
The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee
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Immunogen
Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
Database link: O00370 -
Positive control
- induced bacterially expressed human ORF2
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General notes
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.ab106004 is intended for use in studying control of L1 retrotransposons.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading... -
Purity
IgY fraction -
Purification notes
ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer. -
Clonality
Polyclonal -
Isotype
IgY -
Research areas
Images
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Western blot - Anti-LINE-1 ORF2p antibody (ab106004)All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2
Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDaNote: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.