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Anti-LGR5 antibody [OTI2A2] (ab273092)

Key features and details

  • Mouse monoclonal [OTI2A2] to LGR5
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF, IP
  • Reacts with: Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-LGR5 antibody [OTI2A2]
    See all LGR5 primary antibodies

  • Description

    Mouse monoclonal [OTI2A2] to LGR5

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    Recombinant fragment
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human LGR5 aa 250-550. Genbank: NP_003658)
    Database link: O75473

  • Positive control

    • WB: HEK-293T cells transfected with pCMV6-ENTRY LGR5 cDNA IHC-P: Human lymph node and breast tissue. Human bladder carcinoma, thyroid carcinoma, colon adenocarcinoma and colon adenocarcinoma tissue. Flow: HEK-293T cells transfected with pCMV6-ENTRY LGR5 cDNA ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY LGR5. HT-29 cells.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human lymph node tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Western blot - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Western blot - Anti-LGR5 antibody [OTI2A2] (ab273092)
    All lanes : Anti-LGR5 antibody [OTI2A2] (ab273092) at 1/2000 dilution

    Lane 1 : HEK-293T cells were transfected with the pCMV6ENTRY control (Left lane)
    Lane 2 : HEK-293T cells were transfected with pCMV6-ENTRY LGR5, Right lane) cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 99 kDa

  • Immunoprecipitation - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunoprecipitation - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunoprecipitation (IP) of LGR5 by using ab273092 (lane 2) compared against competitive anti-LGR5 antibodies.

    Lane 1: Negative control: IP without adding anti-LGR5 antibody.

    Lane 2: ab273092 IP.

    Lanes 3-8: Competitior anti-LGR5 antibodies.

    For each experiment, 500ul of DDK tagged LGR5 overexpression lysates (at 1/5 dilution with HEK-293T lysate), 2ug of anti-LGR5 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immunoprecipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

  • Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Flow Cytometry - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Flow cytometric analysis of HEK-293T cells transfected with either LGR5 overexpressed plasmid (Red) or empty vector control plasmid (Blue). Cells were immunostained with ab273092 at 1/100 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunofluorescent staining of HT-29 cells labeling LGR5 using ab273092 at 1/100 dilution (green). Actin filaments were labeled with TRITC-phalloidin (red). The nuclear counter stain is DAPI (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human bladder carcinoma tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human thyroid carcinoma tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human colon adenocarcinoma tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human colon tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunohistochemical staining of paraffin embedded human breast adenocarcinoma tissue labeling LGR5 using ab273092 at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)
    Immunocytochemistry/ Immunofluorescence - Anti-LGR5 antibody [OTI2A2] (ab273092)

    Immunofluorescent staining of COS-7 cells transiently transfected by pCMV6-ENTRY LGR5, labeling LGR5 using ab273092 at 1/100 dilution.

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