Anti-LC3B antibody [RM293] (ab222776)
![Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282653-anti-lc3b-antibody-rm293-western-blot.jpg "Anti-LC3B antibody [RM293] (ab222776)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM293] to LC3B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-LC3B antibody [RM293]
See all LC3B primary antibodies -
Description
Rabbit monoclonal [RM293] to LC3B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human LC3B (N terminal).
Database link: Q9GZQ8 -
Positive control
- WB: HeLa cell lysate, untreated or treated with chloroquine. IHC-P: Human brain tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab222776 was purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM293 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-LC3B antibody [RM293] (ab222776)All lanes : Anti-LC3B antibody [RM293] (ab222776) at 1/200 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with chloroquine, cell lysate
Predicted band size: 15 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282654-anti-lc3b-antibody-rm293-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LC3B antibody [RM293] (ab222776)Formalin-fixed, paraffin-embedded human brain tissue stained for LC3B with ab222776 at 1/200 dilution in immunohistochemical analysis.
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![Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-282655-anti-lc3b-antibody-rm293-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [RM293] (ab222776)HeLa (human epithelial cell line from cervix adenocarcinoma) cells, untreated (left panel) or treated with chloroquine (right panel) stained for LC3B (red) using ab222776 at 1/200 dilution in ICC/IF.
Actin filaments were labeled with fluorescein phalloidin (green). Nuclei were labeled with DAPI (blue).
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![Anti-LC3B antibody [RM293] (ab222776)](https://www.abcam.com/ps/products/222/ab222776/Images/ab222776-4-benefits-of-recombinant-antibodies.png)
Anti-LC3B antibody [RM293] (ab222776)
