Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LATS2 antibody (ab110780)

Key features and details

  • Rabbit polyclonal to LATS2
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-LATS2 antibody
    See all LATS2 primary antibodies

  • Description

    Rabbit polyclonal to LATS2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in Human Ovary and Human Testis tissue lysates as well as the following whole cell lysates: HeLa; HepG2; MCF7. It also gave a positive signal in FFPE human heart tissue sections.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Chimpanzee
Gorilla
Orangutan
Application Abreviews Notes
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
Notes
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).

Target

  • Function

    Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.

  • Tissue specificity

    Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

  • Sequence similarities

    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 UBA domain.

  • Post-translational
    modifications

    Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.

  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ13161 antibody
      • Kinase phosphorylated during mitosis protein antibody
      • KPM antibody
      • Large tumor suppressor homolog 2 antibody
      • Large tumor suppressor homolog 2 Drosophila antibody
      • Large tumor suppressor kinase 2 antibody
      • LATS large tumor suppressor Drosophila homolog 2 antibody
      • LATS large tumor suppressor homolog 2 antibody
      • Lats2 antibody
      • LATS2_HUMAN antibody
      • Serine/threonine kinase kpm antibody
      • Serine/threonine protein kinase kpm antibody
      • Serine/threonine-protein kinase kpm antibody
      • Serine/threonine-protein kinase LATS2 antibody
      • Warts like kinase antibody
      • Warts-like kinase antibody
      see all

    Images

    • Western blot - Anti-LATS2 antibody (ab110780)
      Western blot - Anti-LATS2 antibody (ab110780)
      All lanes : Anti-LATS2 antibody (ab110780) at 1 µg/ml

      Lane 1 : Human ovary tissue lysate - total protein (ab30222)
      Lane 2 : Human testis tissue lysate - total protein (ab30257)
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 120 kDa
      Observed band size: 120 kDa
      Additional bands at: 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody (ab110780)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody (ab110780)
      IHC image of ab110780 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110780, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (6)

    Publishing research using ab110780? Please let us know so that we can cite the reference in this datasheet.

    ab110780 has been referenced in 6 publications.

    • Wu X  et al. RNA Binding Protein RNPC1 Suppresses the Stemness of Human Endometrial Cancer Cells via Stabilizing MST1/2 mRNA. Med Sci Monit 26:e921389 (2020). PubMed: 32088727
    • Guo C  et al. LATS2 inhibits cell proliferation and metastasis through the Hippo signaling pathway in glioma. Oncol Rep 41:2753-2761 (2019). PubMed: 30896861
    • Sun L  et al. MicroRNA-744 promotes carcinogenesis in osteosarcoma through targeting LATS2. Oncol Lett 18:2523-2529 (2019). PubMed: 31452740
    • Montavon C  et al. Outcome in serous ovarian cancer is not associated with LATS expression. J Cancer Res Clin Oncol 145:2737-2749 (2019). PubMed: 31586262
    • Li Y  et al. Long noncoding RNA DDX11-AS1 epigenetically represses LATS2 by interacting with EZH2 and DNMT1 in hepatocellular carcinoma. Biochem Biophys Res Commun 514:1051-1057 (2019). PubMed: 31097223
    • Sadaf  et al. Hypermethylated LATS2 gene with decreased expression in female breast cancer: A case control study from North India. Gene 676:156-163 (2018). PubMed: 30010037

    Images

    • Western blot - Anti-LATS2 antibody (ab110780)
      Western blot - Anti-LATS2 antibody (ab110780)
      All lanes : Anti-LATS2 antibody (ab110780) at 1 µg/ml

      Lane 1 : Human ovary tissue lysate - total protein (ab30222)
      Lane 2 : Human testis tissue lysate - total protein (ab30257)
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 120 kDa
      Observed band size: 120 kDa
      Additional bands at: 65 kDa, 80 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 20 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody (ab110780)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LATS2 antibody (ab110780)
      IHC image of ab110780 staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab110780, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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