Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23654-127] to LAMC2 - BSA and Azide free
  • Suitable for: WB, ICC, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free
    See all LAMC2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23654-127] to LAMC2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 whole cell lysate. ICC: A431 cells. Flow cyt: A549 cells. IP: A431 whole cell lysate.

  • General notes

    ab274384 is the carrier-free version of ab274376. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    Immunocytochemistry - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A431 cells labelling LAMC2 with ab274376 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line. Low expression control: A549 (PMID: 25591736). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200  dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274376).

  • Western blot - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    Western blot - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    All lanes : Anti-LAMC2 antibody [EPR23654-127] (ab274376) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
    Lane 2 : A549 (human lu carcinoma epithelial cell), whole cell lysate
    Lane 3 : WI-38 (human fetal lu fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 131 kDa
    Observed band size: 140,80 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Bands of 140KDa and 80KDa respectively represent  the full length and endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 25591736, 21829200).

    Low expression: A549 (PMID:25591736) and WI-38 (PMID: 10964684)

    Expsure time: 3 minutes

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274376).

  • Flow Cytometry - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    Flow Cytometry - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell)(Left) A431 (Human epidermoid carcinoma epithelial cell)(Right) cells labelling LAMC2 with ab274376 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Low expression control: A549.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274376).

  • Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    Immunoprecipitation - Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)

    LAMC2 was immunoprecipitated from 0.35 mg A431 (human epidermoid carcinoma epithelial cell), whole cell lysate with ab274376 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274376 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab274376 IP in A431 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab274376 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 41 seconds

    Band of 140KDa represents the full length form, Bands of 100KDa and 80KDa represent the endogenously cleaved form.

    The expression profile observed is consistent with what has been described in the literature (PMID: 21829200).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab274376).

  • Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)
    Anti-LAMC2 antibody [EPR23654-127] - BSA and Azide free (ab274384)

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