Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [CL3087] to LAMA
Suitable for: IHC-P, WB
Knockout validated
Reacts with: Human
Isotype: IgG1

Product name

Anti-LAMA antibody [CL3087]
Description

Mouse monoclonal [CL3087] to LAMA
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human LAMA aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P25391

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Epitope

ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)
Positive control
- WB: HeLa cell lysate; Human testis tissue lysate. IHC-P: Human lymph node and testis tissues.
General notes

This product was previously labelled as Laminin alpha 1

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

CL3087
Isotype

IgG1
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)

Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA using ab210954 at a 1/200 dilution.
Western blot - Anti-LAMA antibody [CL3087] (ab210954)

All lanes : Anti-LAMA antibody [CL3087] (ab210954) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : LAMA1 knockout HeLa cell lysate
Lane 3 : Human testis tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

Predicted band size: 337 kDa
Observed band size: 337 kDa

Lanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.

ab210954 Anti-LAMA antibody [CL3087] was shown to specifically react with LAMA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)

Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA using ab210954 at a 1/200 dilution.
Western blot - Anti-LAMA antibody [CL3087] (ab210954)

All lanes : Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution

Lane 1 : Recombinant protein fragment of human Laminin 111
Lane 2 : Recombinant protein fragment of human Laminin 121
Lane 3 : Recombinant protein fragment of human Laminin 221
Lane 4 : Recombinant protein fragment of human Laminin 332
Lane 5 : Recombinant protein fragment of human Laminin 411
Lane 6 : Recombinant protein fragment of human Laminin 521

Developed using the ECL technique.

Predicted band size: 337 kDa
Western blot - Anti-LAMA antibody [CL3087] (ab210954)

Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate

Developed using the ECL technique.

Predicted band size: 337 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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