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Anti-LAMA antibody [CL3087] (ab210954)

Key features and details

  • Mouse monoclonal [CL3087] to LAMA
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-LAMA antibody [CL3087]

  • Description

    Mouse monoclonal [CL3087] to LAMA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human LAMA aa 600-700. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P25391

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Epitope

    ab210954 binds to an epitope located within the peptide sequence LVPENFQDFHSKRQI (aa 640 - 654)

  • Positive control

    • WB: HeLa cell lysate; Human testis tissue lysate. IHC-P: Human lymph node and testis tissues.

  • General notes

     This product was previously labelled as Laminin alpha 1

     

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)

    Immunohistochemical analysis of paraffin-embedded Human testis tissue, labeling LAMA using ab210954 at a 1/200 dilution.

  • Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    All lanes : Anti-LAMA antibody [CL3087] (ab210954) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LAMA1 knockout HeLa cell lysate
    Lane 3 : Human testis tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution

    Predicted band size: 337 kDa
    Observed band size: 337 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab210954 observed at 337 kDa. Red - loading control ab129002 observed at 124 kDa.

     ab210954 Anti-LAMA antibody [CL3087] was shown to specifically react with LAMA in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264991 (knockout cell lysate ab258937) was used. Wild-type and LAMA knockout samples were subjected to SDS-PAGE. ab210954 and Anti-Vinculin antibody [EPR8185] (ab129002) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMA antibody [CL3087] (ab210954)

    Immunohistochemical analysis of paraffin-embedded Human lymph node tissue (negative control), labeling LAMA using ab210954 at a 1/200 dilution.

  • Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    All lanes : Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution

    Lane 1 : Recombinant protein fragment of human Laminin 111
    Lane 2 : Recombinant protein fragment of human Laminin 121
    Lane 3 : Recombinant protein fragment of human Laminin 221
    Lane 4 : Recombinant protein fragment of human Laminin 332
    Lane 5 : Recombinant protein fragment of human Laminin 411
    Lane 6 : Recombinant protein fragment of human Laminin 521

    Developed using the ECL technique.

    Predicted band size: 337 kDa

  • Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    Western blot - Anti-LAMA antibody [CL3087] (ab210954)
    Anti-LAMA antibody [CL3087] (ab210954) at 1/500 dilution + Human testis tissue lysate

    Developed using the ECL technique.

    Predicted band size: 337 kDa

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