Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase - BSA and Azide free
  • Suitable for: IHC-P, WB, Flow Cyt, IP, ICC
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free
    See all Lactate Dehydrogenase primary antibodies

  • Description

    Rabbit monoclonal [EP1565Y] to Lactate Dehydrogenase - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, Flow Cyt, IP, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab244228 is the carrier-free version of ab53292 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab244228 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/5000 dilution

    Lane 1 : Wild-type Hap1 cell lysate
    Lane 2 : LDHB knockout Hap1 cell lysate
    Lane 3 : Wild-type HEK-293T cell lysate
    Lane 4 : LDHB knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    This data was developed using ab53292, the same antibody clone in a different buffer formulation.

    Lanes 1 - 4: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007 observed at 125 kDa.

    ab53292 was shown to react with Lactate Dehydrogenase in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255403 (knockout cell lysate ab263761) was used. Wild-type and Lactate Dehydrogenase knockout samples were subjected to SDS-PAGE. ab53292 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    All lanes : Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) at 1/50000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : LDHB (Lactate Dehydrogenase B) knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 37 kDa



    This data was developed using ab53292, the same antibody clone in a different buffer formulation.

    Lanes 1 - 3: Merged signal (red and green). Green - ab53292 observed at 37 kDa. Red - loading control, ab130007, observed at 130 kDa.

    ab53292 was shown to specifically react with Lactate Dehydrogenase B in wild-type HAP1 cells as signal was lost in LDHB (Lactate Dehydrogenase B) knockout cells. Wild-type and LDHB (Lactate Dehydrogenase B) knockout samples were subjected to SDS-PAGE. ab53292 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/50000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Immunocytochemistry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    This data was developed using ab53292, the same antibody clone in a different buffer formulation.ICC/IF image of ab53292 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53292, neat) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    This data was developed using ab53292, the same antibody clone in a different buffer formulation.Human heart muscle labeled with ab53292 at 1/100 - 1/250 dilution Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Western blot - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Anti-Lactate Dehydrogenase B/LDH-B antibody [EP1565Y] (ab53292) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP

    Predicted band size: 37 kDa
    Observed band size: 37 kDa



    This data was developed using ab53292, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Flow Cytometry - Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    This data was developed using ab53292, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab53292 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53292, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)
    Anti-Lactate Dehydrogenase antibody [EP1565Y] - BSA and Azide free (ab244228)

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