Anti-KLF4 antibody (ab272860)
Key features and details
- Rabbit polyclonal to KLF4
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-KLF4 antibody
See all KLF4 primary antibodies -
Description
Rabbit polyclonal to KLF4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human KLF4 (C terminal). The exact sequence is proprietary.
Database link: O43474 -
Positive control
- Flow Cyt: Human embryonic stem cells (ESC). WB: HEK-293T, A431, HeLa, HepG2, HCT 116, Rat2, NIH/3T3 whole cell lysate and mouse and human ESC. IP: HeLa whole cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Flow Cytometry - Anti-KLF4 antibody (ab272860)Flow cytometry analysis of human embryonic stem cells labeling KLF4 using ab272860 at 1:100 dilution (purple) or rabbit IgG (black).
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/2000 dilution
Lane 1 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 2 : HCT 116 nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-Synaptojanin antibody (ab272560) at 1/5000 dilution + Rat2 (rat fibroblast cell line) whole cell lysate
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/5000 dilution + NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Mouse embryonic stem cells at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody12% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)Anti-KLF4 antibody (ab272860) at 1/3000 dilution + Human embryonic stem cells at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-KLF4 antibody (ab272860)All lanes : Anti-KLF4 antibody (ab272860) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2 : Transfected HEK-293 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunoprecipitation - Anti-KLF4 antibody (ab272860)10% SDS-PAGE.
Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 μg of ab272860.
All lanes : Anti-KLF4 antibody (ab272860) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Control IgG
Lane 3 : Immunoprecipitation of KLF4 protein from HeLa whole cell extracts using 5 µg of ab272860
Secondary
All lanes : anti Rabbit IgG (HRP)