Anti-KIAA0513 antibody (ab121382)
Key features and details
- Rabbit polyclonal to KIAA0513
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-KIAA0513 antibody
See all KIAA0513 primary antibodies -
Description
Rabbit polyclonal to KIAA0513 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 134-271 of Human KIAA0513.
-
Positive control
- KIAA0513-transfected HEK293T lysate; Human cerebellum tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-KIAA0513 antibody (ab121382)All lanes : Anti-KIAA0513 antibody (ab121382) at 1/250 dilution
Lane 1 : vector only transfected HEK293T lysate
Lane 2 : KIAA0513-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
10-20% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KIAA0513 antibody (ab121382)Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli. Recommended concentration of ab121382 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIAA0513 antibody (ab121382)ab121382 at 1/500 dilution staining KIAA0513 in Paraffin-embedded Human cerebellum tissue by Immunohistochemistry. Note: strong cytoplasmic and nuclear positivity in Purkinje cells and strong cytoplasmic positivity in cells in granular layer and molecular layer.
