Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-Kappa Opioid Receptor antibody [EPR18881]
    See all Kappa Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysates. IHC-P: Mouse and rat hippocampus tissues. IP: Mouse brain whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18881

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

  • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat heart lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat spleen lysate
    Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 43 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Mainly membrane staining on neuron cell of mouse hippocampus is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on mouse spleen.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Mainly membrane staining on neuron cells of rat hippocampus is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative staining on rat spleen.

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Kappa Opioid Receptor was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab183825 at 1/100 dilution.

    Western blot was performed from the immunoprecipitate using ab183825 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Mouse brain whole cell lysate 10µg (Input).

    Lane 2: ab183825 IP in Mouse brain whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183825 in Mouse brain whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

  • Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
    Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

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