Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12855(B)] to K31 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-K31 antibody [EPR12855(B)] - BSA and Azide free
    See all K31 primary antibodies

  • Description

    Rabbit monoclonal [EPR12855(B)] to K31 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human skin lysate IHC-P: Human skin tissue.

  • General notes

    Ab250025 is the carrier-free version of ab177955. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250025 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12855(B)

  • Isotype

    IgG

Images

  • Western blot - Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)
    Western blot - Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)
    Anti-K31 antibody [EPR12855(B)] (ab177955) at 1/1000 dilution (Purified) + Human skin lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177955).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin tissue sections labeling K31 with Purified ab177955 at 1/1000 dilution (0.55 µg/mL). Heat mediated antigen retrieval using Bond™™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab177955).

  • Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)
    Anti-K31 antibody [EPR12855(B)] - BSA and Azide free (ab250025)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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