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Developmental Biology Reproduction Fertilisation

Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)

Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [125] to IZUMO1 - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-IZUMO1 antibody [125] - BSA and Azide free
    See all IZUMO1 primary antibodies
  • Description

    Rat monoclonal [125] to IZUMO1 - BSA and Azide free
  • Host species

    Rat
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Tissue, cells or virus corresponding to Mouse IZUMO1. Mouse sperm

  • Positive control

    • WT mouse sperm lysate; mouse testis and sperm lysates; human testis and sperm lysates; mouse spermatozoa.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Constituents: 50% PBS, 50% Glycerol (glycerin, glycerine)

    filter sterilized
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Ion Exchange Chromatography
  • Clonality

    Monoclonal
  • Clone number

    125
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Developmental Biology
    • Reproduction
    • Fertilisation

Images

  • Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/1000 dilution

    Lane 1 : WT mouse sperm lysate
    Lane 2 : IZUMO1 Knock out mouse sperm lysate

    Lysates/proteins at 10 µg per lane.
  • Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/1000 dilution

    Lane 1 : mouse testis lysate
    Lane 2 : mouse sperm lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution


    SDS Page 10-20% gradient gel.

  • Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    Western blot - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    All lanes : Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626) at 1/100 dilution

    Lane 1 : human testis lysate
    Lane 2 : human sperm lysate

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab97057) at 1/10000 dilution
  • Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)
    Immunocytochemistry/ Immunofluorescence - Anti-IZUMO1 antibody [125] - BSA and Azide free (ab211626)

    Immunofluorescent analysis of mouse spermatozoa labeling IZUMO1 using ab211626 at 4 μg/mL. An Alexa Fluor 488 conjugated anti-rat IgG was used as secondary antibody.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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