Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2
Suitable for: WB, IHC-Fr, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ITLN1 + ITLN2 antibody [EPR20311]
See all ITLN1+ITLN2 primary antibodies
Description

Rabbit monoclonal [EPR20311] to ITLN1 + ITLN2
Host species

Rabbit
Specificity

ITLN1 mRNA expression in stomach has been reported [PMID: 19549487]. Another report [PMID: 22768319] shows no staining on stomach at protein level. In our hands this antibody showed weak cytoplasmic staining on stomach samples.

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human ITLN1 DDDDK tagged recombinant protein (aa17-313); Human ITLN2 His-tagged recombinant protein (aa27-325); Human, mouse and rat colon and small intestine lysates. IHC-P: Human, mouse and rat colon tissues. IHC-Fr: Mouse colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20311
Isotype

IgG
Research areas

Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/1000 dilution

Lane 1 : Recombinant human ITLN1 protein (ab109151)
Lane 2 : Human ITLN2 His-tagged recombinant protein (aa27-325)

Lysates/proteins at 0.01 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 36,37 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Frozen sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on goblet cells of human colon is observed [PMID: 22768319] [PMID: 19549487].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on goblet cells of mouse colon is observed [PMID: 22768319] [PMID: 19549487].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling ITLN1 + ITLN2 with ab210483 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on goblet cells of rat colon is observed [PMID: 22768319] [PMID: 19549487].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

Lane 1 : Human colon lysate
Lane 2 : Human fetal spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

Spleen is a negative control (PMID: 11181563).
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

All lanes : Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution

Lane 1 : Mouse colon lysate
Lane 2 : Rat small intestine lysate
Lane 3 : Mouse small intestine lysate
Lane 4 : Rat colon lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1/2: 1 minute; Lane 3/4: 30 seconds.

The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).
Western blot - Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483) at 1/5000 dilution + Human small intestine lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The observed molecular weight is consistent with the literature (PMID: 11313366, PMID: 20628387).
Anti-ITLN1 + ITLN2 antibody [EPR20311] (ab210483)

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