Anti-ITIH2/SHAP antibody (ab110822)
Key features and details
- Rabbit polyclonal to ITIH2/SHAP
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ITIH2/SHAP antibody
See all ITIH2/SHAP primary antibodies -
Description
Rabbit polyclonal to ITIH2/SHAP -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee
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Immunogen
Synthetic peptide corresponding to ITIH2/SHAP.
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Positive control
- Recombinant Human ITIH1/SHAP protein (ab114721) can be used as a positive control in WB. Human OA chondrocyte tissue, mouse OA periosteum tissue ICC/IF: HepG2 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (ab110822)
ab110822 staining ITIH2/SHAP in Human OA chondrocyte tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH2/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
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Immunocytochemistry/ Immunofluorescence - Anti-ITIH2/SHAP antibody (ab110822)Immunofluorescence analysis of ITIH1 was performed using 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab110822 at 2µg/mLin 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Rhodamine Phalloidin (1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (ab110822)ab110822 staining ITIH2/SHAP in Human OA chondrocyte tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH2/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITIH2/SHAP antibody (ab110822)ab110822 staining ITIH2/SHAP in Mouse OA periosteum tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. ITIH2/SHAP is stained in red, hyaluronan in green, and the nuclei in blue.
