Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free (ab251413)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17138-29] to ITIH1/SHAP - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
-
Product name
Anti-ITIH1/SHAP antibody [EPR17138-29] - BSA and Azide free
See all ITIH1/SHAP primary antibodies -
Description
Rabbit monoclonal [EPR17138-29] to ITIH1/SHAP - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab251413 is the carrier-free version of ab204513. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251413 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
EPR17138-29 -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteThis data was developed using ab204513, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/10000 dilution + Human brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab204513, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondThis data was developed using ab204513, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
Anti-ITIH1/SHAP antibody [EPR17138-29] (ab204513) at 1/50000 dilution + Human serum lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 101, 86 kDa
Observed band size: 125,250,80 kDa why is the actual band size different from the predicted?
Exposure time: 2 secondsThis data was developed using ab204513, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-