Anti-ISY1 antibody (ab241583)
Key features and details
- Rabbit polyclonal to ISY1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ISY1 antibody
See all ISY1 primary antibodies -
Description
Rabbit polyclonal to ISY1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP Human
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Immunogen
Synthetic peptide within Human ISY1 aa 200-250. The exact sequence is proprietary.
Database link: Q9ULR0 -
Positive control
- IP: HEK-293T whole cell lysate.
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General notes
ab241583 has not performed satisfactorily when used for WB of ISY1 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of ISY1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to ISY1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-ISY1 antibody (ab241583)
ISY1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241583 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241583 at 0.4 µg/ml.
Lane 1: ab241583 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
