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Anti-IRAK4 antibody [2H9] (ab119942)

Key features and details

  • Mouse monoclonal [2H9] to IRAK4
  • Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human, African green monkey, Recombinant fragment
  • Isotype: IgG1

Overview

  • Product name

    Anti-IRAK4 antibody [2H9]
    See all IRAK4 primary antibodies

  • Description

    Mouse monoclonal [2H9] to IRAK4

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4

  • Positive control

    • Recombinant IRAK4 protein; THP-1, Hela, K562, MCF7, RAW264.7, Jurkat and Cos7 cell lysates; Human lung cancer amd kidney cancer tissues; Hela cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

Properties

Images

  • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
    Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
    All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution

    Lane 1 : THP-1 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : MCF7 cell lysate
    Lane 5 : RAW264.7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

    Predicted band size: 52 kDa

  • Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
    Western blot - Anti-IRAK4 antibody [2H9] (ab119942)
    Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

    Predicted band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
    ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)
    ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining.
  • Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
    Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)
    Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red).
  • ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
    ELISA - Anti-IRAK4 antibody [2H9] (ab119942)
    ELISA using ab119942.
  • Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)
    Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)

    ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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