Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Mouse monoclonal [2H9] to IRAK4
Suitable for: WB, ELISA, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Human, African green monkey, Recombinant fragment
Isotype: IgG1

Product name

Anti-IRAK4 antibody [2H9]
See all IRAK4 primary antibodies
Description

Mouse monoclonal [2H9] to IRAK4
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Recombinant fragment, corresponding to amino acids 21-198 of Human IRAK4
Positive control
- Recombinant IRAK4 protein; THP-1, Hela, K562, MCF7, RAW264.7, Jurkat and Cos7 cell lysates; Human lung cancer amd kidney cancer tissues; Hela cells This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HepG2.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage buffer

Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Protein G purified
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

2H9
Isotype

IgG1
Research areas

Western blot - Anti-IRAK4 antibody [2H9] (ab119942)

All lanes : Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution

Lane 1 : THP-1 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : RAW264.7 cell lysate
Lane 6 : Jurkat cell lysate
Lane 7 : COS-7 (African green monkey kidney fibroblast-like cell line) cell lysate

Predicted band size: 52 kDa
Western blot - Anti-IRAK4 antibody [2H9] (ab119942)

Anti-IRAK4 antibody [2H9] (ab119942) at 1/500 dilution + Recombinant IRAK4 protein

Predicted band size: 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)

ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human lung cancer tissue by Immunohistochemistry with DAB staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [2H9] (ab119942)

ab119942, at 1/200 dilution, staining IRAK4 in paraffin-embedded Human kidney cancer tissue by Immunohistochemistry with DAB staining.
Flow Cytometry - Anti-IRAK4 antibody [2H9] (ab119942)

Flow cytometric analysis of Hela cells using ab119942 at 1/200 dilution (blue) and negative control (red).
ELISA - Anti-IRAK4 antibody [2H9] (ab119942)

ELISA using ab119942.
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [2H9] (ab119942)

ab119942 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab119942 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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