Anti-INTS5 antibody (ab74405)
Key features and details
- Rabbit polyclonal to INTS5
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-INTS5 antibody
See all INTS5 primary antibodies -
Description
Rabbit polyclonal to INTS5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 1000 and 1050 of human INTS5 (NP_085131.1)
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Positive control
- HeLa and NIH3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74405 was affinity purified using an epitope specific to INTS5 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-INTS5 antibody (ab74405)All lanes : Anti-INTS5 antibody (ab74405) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Observed band size: 108 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
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Immunoprecipitation - Anti-INTS5 antibody (ab74405)Detection of INTS5 by Western Blot of Immunprecipitate.
ab74405, at 1µg/ml, staining INTS5 in HeLa whole cell lysate immunoprecipitated using ab74405 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
