Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23341-7] to Interferon beta - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free
    See all Interferon beta primary antibodies

  • Description

    Rabbit monoclonal [EPR23341-7] to Interferon beta - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant Human IFN-ß protein; A549 (transfected with 400 ng/ml poly(I:C) for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection) whole cell lysate. IP: A549 (transfected with 400 ng/ml poly(I:C) for 24 hours, 300ng/ml BFA was added to cells 4 hours after the start of the transfection) whole cell lysate.

  • General notes

    ab275889 is the carrier-free version of ab275880. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab275889 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    Western blot - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    All lanes : Anti-Interferon beta antibody [EPR23341-7] (ab275880) at 1/1000 dilution

    Lane 1 : A549 (human lu carcinoma epithelial cell) transfected with 400/ml poly(I:C) for 24 hours, 300/ml BFA was added to cells 4 hours after the start of the transfection, whole cell lysate
    Lane 2 : A549 (human lu carcinoma epithelial cell) transfected with water instead of poly I:C for 24 hours, 300/ml BFA was added to cells 4 hours after the start of the transfection, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab275880, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight  observed is consistent with what has been described in the literature (PMID:7352261).

    Exposure time: 97 seconds.

  • Western blot - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    Western blot - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    Anti-Interferon beta antibody [EPR23341-7] (ab275880) at 1/1000 dilution + Recombinant Human IFN-beta protein, 10 ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 22 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab275880, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 3 seconds.

  • Immunoprecipitation - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    Immunoprecipitation - Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)

    This data was developed using ab275880, the same antibody clone in a different buffer formulation.

    Interferon beta was immunoprecipitated from 0.35 mg A549 (human lung carcinoma epithelial cell) (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate  with ab275880 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275880 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate 10 ug

    Lane 2: ab275880 IP in A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275880 in A549 (transfected with 400ng/ml poly(I:C) for 4 hours, then 300ng/ml BFA was added for the last 20 hours) whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

  • Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)
    Anti-Interferon beta antibody [EPR23341-7] - BSA and Azide free (ab275889)

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