Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)
![Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)](https://www.abcam.com/ps/products/273/ab273669/Images/ab273669-394735-AntiInsulindegradingenzymeIDEantibodyOTI4E5westernblothela-ko-human.jpg "Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)")
Key features and details
- Mouse monoclonal [OTI4E5] to Insulin degrading enzyme / IDE - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free
See all Insulin degrading enzyme / IDE primary antibodies -
Description
Mouse monoclonal [OTI4E5] to Insulin degrading enzyme / IDE - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHumanAfrican green monkey
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Immunogen
Recombinant fragment corresponding to Human Insulin degrading enzyme/ IDE aa 800 to the C-terminus. (NP_004960) produced in E.coli.
Database link: P14735 -
Positive control
- WB: SVT2, A549, HeLa, COS-7 and HeLa cell extracts. Wild type HeLa cell lysate. HEK-293T cells transfected with pCMV6-ENTRY IDE cDNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI4E5 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)All lanes : Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1 : Wild type HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : IDE knock out HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 118 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)](https://www.abcam.com/ps/products/273/ab273669/Images/ab273669-394433-AntiInsulindegradingenzymeIDEantibodyOTI4E5westernblotSVT2A549HeLaCOS7human.jpg)
Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)All lanes : Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extract
Lane 2 : SVT2 (Mouse cell line) cell extract
Lane 3 : A549 (Human lung carcinoma cell line) cell extract
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extract
Lysates/proteins at 35 µg per lane.
Predicted band size: 118 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.
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![Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)](https://www.abcam.com/ps/products/273/ab273669/Images/ab273669-394436-AntiInsulindegradingenzymeIDEantibodyOTI4E5westernblottransfectedHEK293T.jpg)
Western blot - Anti-Insulin degrading enzyme / IDE antibody [OTI4E5] - BSA and Azide free (ab273669)All lanes : Same antibody clone in a different buffer formulation at 1/500 dilution
Lane 1 : HEK-293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IDE cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 118 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide.