Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3425(2)] to INPP4A - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free
    See all INPP4A primary antibodies

  • Description

    Rabbit monoclonal [EP3425(2)] to INPP4A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247910 is the carrier-free version of ab109622. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247910 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)

    This data was developed using ab109622, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: INPP4A knockout HAP1 cell lysate (20 µg)

    Lane 3: Jurkat cell lysate (20 µg)

    Lane 4: Human fetal brain tissue lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109622 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109622 was shown to specifically react with INPP4A when INPP4A knockout samples were used, along with additional cross-reactive bands. Wild-type and INPP4A knockout samples were subjected to SDS-PAGE. ab109622 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    Western blot - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    All lanes : Anti-INPP4A antibody [EP3425(2)] (ab109622) at 1/1000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 110 kDa
    Observed band size: 104 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab109622, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    This data was developed using ab109622, the same antibody clone in a different buffer formulation.ab109622 at 1/100 dilution staining INPP4A in paraffin-embedded Human brain tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)
    Anti-INPP4A antibody [EP3425(2)] - BSA and Azide free (ab247910)

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