Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [7A11] to ING5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ING5 antibody [7A11] - BSA and Azide free
    See all ING5 primary antibodies

  • Description

    Rat monoclonal [7A11] to ING5 - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human ING5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q8WYH8

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Jurkat cell nuclear fraction; MCF7, Jurkat, A549, HT-1080, HepG2 and HEK-293 whole cell lysates.

  • General notes

    ab255832 is the carrier-free version of ab252838. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255832 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)
    Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)
    All lanes : Anti-ING5 antibody [7A11] (ab252838) at 0.66 µg/ml

    Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
    Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 6 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252838).

  • Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)
    Western blot - Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)
    All lanes : Anti-ING5 antibody [7A11] (ab252838) at 0.66 µg/ml

    Lane 1 : Jurkat cytoplasmic fraction
    Lane 2 : Jurkat nuclear fraction

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 28 kDa
    Observed band size: 28 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252838).

  • Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)
    Anti-ING5 antibody [7A11] - BSA and Azide free (ab255832)

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