Anti-ING5 antibody [7A11] (ab252838)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [7A11] to ING5
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-ING5 antibody [7A11]
See all ING5 primary antibodies -
Description
Rat monoclonal [7A11] to ING5 -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
Recombinant fragment within Human ING5. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q8WYH8 -
Positive control
- WB: Jurkat cell nuclear fraction; MCF7, Jurkat, A549, HT-1080, HepG2 and HEK-293 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7A11 -
Isotype
IgG2a -
Research areas
Images
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All lanes : Anti-ING5 antibody [7A11] (ab252838) at 0.66 µg/ml
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 4 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate
Lane 5 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 6 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-ING5 antibody [7A11] (ab252838) at 0.66 µg/ml
Lane 1 : Jurkat cytoplasmic fraction
Lane 2 : Jurkat nuclear fraction
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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