Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free
    See all Indoleamine 2, 3-dioxygenase primary antibodies

  • Description

    Rabbit monoclonal [EPR24032-22] to Indoleamine 2, 3-dioxygenase - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: C2C12 (treated with 50ng/ml IFN-gamma (mouse) for 24 hours) whole cell lysate; Mouse colon tissue lysate. IP: Mouse colon tissue lysate.

  • General notes

    ab277531 is the carrier-free version of ab277522. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab277531 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    All lanes : Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution

    Lane 1 : C2C12 (mouse myoblasts myoblast), whole cell lysate
    Lane 2 : C2C12 treated with 50ng/ml IFN-gamma (mouse) for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 46 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab277522, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 30809284; PMID: 19741271).

    Exposure time: 26 seconds.

     

  • Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    Immunoprecipitation - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)

    This data was developed using ab277522, the same antibody clone in a different buffer formulation.

    Indoleamine 2,3-dioxygenase was immunoprecipitated from 0.35 mg Mouse colon tissue lysate with ab277522 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277522 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse colon tissue lysate 10µg

    Lane 2: ab277522 IP in mouse colon tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277522 in mouse colon tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

  • Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    Western blot - Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] (ab277522) at 1/1000 dilution + Mouse colon tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 46 kDa why is the actual band size different from the predicted?



    This data was developed using ab277522, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression pattern is consistent with what has been described in the literature (PMID: 19741271).

    Exposure time: 5.5 seconds.

  • Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)
    Anti-Indoleamine 2, 3-dioxygenase antibody [EPR24032-22] - BSA and Azide free (ab277531)

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