Anti-IMPA2 antibody [EPR23668-64] (ab256410)
![Anti-IMPA2 antibody [EPR23668-64] (ab256410)](https://www.abcam.com/ps/products/256/ab256410/Images/ab256410-455475-anti-impa2-antibody-epr2366864-western-blot-adenocarcinoma.jpg "Anti-IMPA2 antibody [EPR23668-64] (ab256410)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23668-64] to IMPA2
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-IMPA2 antibody [EPR23668-64]
See all IMPA2 primary antibodies -
Description
Rabbit monoclonal [EPR23668-64] to IMPA2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Recombinant fragmentWB MouseHumanRecombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-468, HeLa, RAW264.7, PC-12 and MCF7 transfected with IMPA2 expression vector lysates. IP: MDA-MB-468 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23668-64 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IMPA2 antibody [EPR23668-64] (ab256410)All lanes : Anti-IMPA2 antibody [EPR23668-64] (ab256410) at 1/1000 dilution
Lane 1 : MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The observed MW is consistent with what has been described in the literature (PMID: 23205734).
Exposure time: Lanes 1-2: 3 minutes; Lanes 3-4: 81 seconds.
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![Immunoprecipitation - Anti-IMPA2 antibody [EPR23668-64] (ab256410)](https://www.abcam.com/ps/products/256/ab256410/Images/ab256410-3-anti-impa2-antibody-epr2366864-immunoprecipitation-mdamb468.jpg)
Immunoprecipitation - Anti-IMPA2 antibody [EPR23668-64] (ab256410)IMPA2 was immunoprecipitated from 0.35 mg MDA-MB-468 (human breast adenocarcinoma epithelial cell), whole cell lysate 10 µg with ab256410 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256410 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MDA-MB-468 whole cell lysate 10 µg
Lane 2: ab256410 IP in MDA-MB-468 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256410 in MDA-MB-468 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 24 seconds
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![Western blot - Anti-IMPA2 antibody [EPR23668-64] (ab256410)](https://www.abcam.com/ps/products/256/ab256410/Images/ab256410-455476-anti-impa2-antibody-epr2366864-western-blot-adenocarcinoma1.jpg)
Western blot - Anti-IMPA2 antibody [EPR23668-64] (ab256410)All lanes : Anti-IMPA2 antibody [EPR23668-64] (ab256410) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma epithelial cell) transfected with IMPA2 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 31 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Lysates were kindly provided by an anonymous collaborator.
Exposure time: 3.25 seconds.
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![Anti-IMPA2 antibody [EPR23668-64] (ab256410)](https://www.abcam.com/ps/products/256/ab256410/Images/ab256410-4-Benefits-of-Recombinant-antibodies.jpg)
Anti-IMPA2 antibody [EPR23668-64] (ab256410)
