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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)

Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12022] to IMP3 - BSA and Azide free
  • Suitable for: ICC, IP, WB
  • Knockout validated
  • Reacts with: Rat, Human

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Overview

  • Product name

    Anti-IMP3 antibody [EPR12022] - BSA and Azide free
    See all IMP3 primary antibodies
  • Description

    Rabbit monoclonal [EPR12022] to IMP3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IP, WBmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant fragment within Human IMP3. The exact sequence is proprietary.
    Database link: O00425

  • General notes

    ab250015 is the carrier-free version of ab177942 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250015 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12022
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding
    • Cancer
    • Tumor biomarkers
    • Hormones

Images

  • Western blot - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    Western blot - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)

    This data was developed using ab177942, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: HEK293 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab177942 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab177942 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. ab177942 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    Immunocytochemistry - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    This data was developed using ab177942, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labeling IMP3 with ab177942 at 1/50 dilution (green). DAPI nuclear staining (blue).
  • Immunoprecipitation - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    Immunoprecipitation - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    This data was developed using ab177942, the same antibody clone in a different buffer formulation.Western blot analysis on immunoprecipitation pellet from 293T cell lysate using ab177942.
  • Western blot - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    Western blot - Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    All lanes : Anti-IMP3 antibody [EPR12022] (ab177942) at 1/1000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HT-29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 63 kDa
    Observed band size: 69 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab177942, the same antibody clone in a different buffer formulation.

     

  • Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)
    Anti-IMP3 antibody [EPR12022] - BSA and Azide free (ab250015)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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