Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)
![Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-6-anti-il36-alphail1f6-antibody-epr2308987-western-blot-lysate.jpg "Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23089-87] to IL36 alpha/IL-1F6
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87]
See all IL36 alpha/IL-1F6 primary antibodies -
Description
Rabbit monoclonal [EPR23089-87] to IL36 alpha/IL-1F6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: Human tonsil tissue lysate. IHC-P: Human psoriatic skin. Human tonsil tissue. Human colon carcinoma tissue. WB: Human tonsil tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23089-87 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271) at 1/1000 dilution + Human tonsil tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 18 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-3-anti-il36-alphail1f6-antibody-epr2308987-immunohistochemistry-human-psoriatic-skin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)Immunohistochemical analysis of paraffin-embedded human psoriatic skin tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human psoriatic skin is observed (PMID: 28043870). The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-4-anti-il36-alphail1f6-antibody-epr2308987-immunohistochemistry-human-skin.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)Immunohistochemical analysis of paraffin-embedded human skin tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Negative control: No staining on human healthy skin (PMID: 28043870).
The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-5-anti-il36-alphail1f6-antibody-epr2308987-immunoprecipitation-human-tonsil-tissue-lysate-10-ug.jpg)
Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)IL36 alpha/IL-1F6 was immunoprecipitated from 0.35 mg human tonsil tissue lysate with ab269271 at 1/30. Western blot was performed on the immunoprecipitate using ab269271 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human tonsil tissue lysate.
Lane 2: ab269271 IP in human tonsil tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269271 in human tonsil tissue lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 24 secs.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-2-anti-il36-alphail1f6-antibody-epr2308987-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on tonsil epithelium is observed. The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-1-anti-il36-alphail1f6-antibody-epr2308987-immunohistochemistry-human-colon-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling IL36 alpha/IL-1F6 with ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human colon carcinoma tissue is observed (PMID: 30452921). The section was incubated with ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)](https://www.abcam.com/ps/products/269/ab269271/Images/ab269271-7-benefits-of-recombinant-antibodies.png)
Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (ab269271)
