Anti-IL28RA antibody (ab224395)
Key features and details
- Rabbit polyclonal to IL28RA
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-IL28RA antibody
See all IL28RA primary antibodies -
Description
Rabbit polyclonal to IL28RA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human IL28RA aa 100-250.
Database link: Q8IU57 -
Positive control
- IHC-P: Human duodenum, small intestine, uterine cervix and Fallopian tube tissue. ICC/IF: U-251 MG cells. WB: CACO-2 and MCF-2 whole cell lysates.
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General notes
This product was previously labelled as IL-28 Receptor alpha
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-IL28RA antibody (ab224395) at 0.4 µg/ml
Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 58 kDa
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Immunohistochemical analysis of human uterine cervix tissue labeling IL28RA in the cytoplasm and membrane of squamous epithelial cells with ab224395 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent analysis of U-251 MG (human brain glioma cell line) cells labeling IL28 (green) in cytosol with ab224395 at 2 µg/ml.
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Immunohistochemical analysis of human small intestine tissue labeling IL28RA in the cytoplasm and membrane of glandular cells with ab224395 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human duodenum tissue labeling IL28RA in the cytoplasm and membrane of glandular cells with ab224395 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of human Fallopian tube tissue labeling IL28RA in the cytoplasm and membrane of glandular cells with ab224395 at 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.