Anti-IL22 RA2/IL-22BP antibody (ab203211)
Key features and details
- Rabbit polyclonal to IL22 RA2/IL-22BP
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IL22 RA2/IL-22BP antibody
See all IL22 RA2/IL-22BP primary antibodies -
Description
Rabbit polyclonal to IL22 RA2/IL-22BP -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human IL22 RA2/IL-22BP aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q969J5 -
Positive control
- IHC-P: Mouse brain Tissue WB: Mouse spleen and rat testis tissue lysate Flow Cyt: Mouse splenocytes and spleen cells.
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General notes
Protein previously labeled as IL22 RA2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.01% BSA
Aqueous buffered solution. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL22 RA2/IL-22BP antibody (ab203211)Anti-IL22 RA2/IL-22BP antibody (ab203211) at 1/300 dilution + Mouse spleen tissue lysates
Secondary
Conjugated secondary antibody at 1/10000 dilution
Predicted band size: 31 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL22 RA2/IL-22BP antibody (ab203211)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse brain tissue labeling IL22 RA2 / IL-22BP with ab203211 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-IL22 RA2/IL-22BP antibody (ab203211)Anti-IL22 RA2/IL-22BP antibody (ab203211) at 1/300 dilution + Rat testis tissue lysates
Predicted band size: 31 kDaPrimary incubation: overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
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Flow Cytometry - Anti-IL22 RA2/IL-22BP antibody (ab203211)Flow Cytometry analysis of Mouse splenocytes labeling IL22 RA2 / IL-22BP with ab203211 at 1/100, followed by incubation with a PE conjugated secondary antibody (green). Blue represents control cells, secondary only control in shown in light blue and isotype control in orange.
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Flow Cytometry - Anti-IL22 RA2/IL-22BP antibody (ab203211)Flow Cytometry analysis of mouse spleen cells labeling IL22 RA2 / IL-22BP with ab203211 at 1/200, followed by incubation with a goat anti-rabbit PE conjugated secondary antibody (green). Cells were fixed with 2% PFA (10min at room temperature) and then permeabilized with 90% ice-cold methanol for 30min on ice. The cells were then incubated in 2% BSA + 10% goat serum to block non-specific protein-protein interactions for 15 min at room temperature. Dark blue represents control cells, secondary only control in shown in light blue and isotype control in orange.
