Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)
![Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)](https://www.abcam.com/ps/products/231/ab231891/Images/ab231891-302560-rabmab2.jpg "Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21776] to IL-17A - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-IL-17A antibody [EPR21776] - BSA and Azide free
See all IL-17A primary antibodies -
Description
Rabbit monoclonal [EPR21776] to IL-17A - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: EL4 cells treated with PMA, ionomycin and Brefeldin A, whole cell lysate.
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General notes
Ab231891 is the carrier-free version of ab218013. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab231891 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21776 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)All lanes : Anti-IL-17A antibody [EPR21776] (ab218013) at 1/1000 dilution
Lane 1 : Untreated EL4 (mouse lymphoma T lymphocyte) whole cell lysate
Lane 2 : EL4 treated with 50 ng/ml Phorbol-12-myristate-13-acetate (PMA) and 500 ng/ml ionomycin (ab120116) for 6 hours, then with 500 ng/ml Brefeldin A for 18 hours, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 17 kDa
Observed band size: 14,17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/dilution buffer and concentration: 5% NFDM/TBST
Expression of IL17A can be induced by PMA and Ionomycin treatment (PMID 28382171).
The expression profile is consistent with the literature (PMID 9764847).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218013).
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![Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)](https://www.abcam.com/ps/products/231/ab231891/Images/ab231891-2-benefits-of-recombinant-antibodies.png)
Anti-IL-17A antibody [EPR21776] - BSA and Azide free (ab231891)
