Anti-IKZF4 antibody (ab155754)
Key features and details
- Rabbit polyclonal to IKZF4
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-IKZF4 antibody
See all IKZF4 primary antibodies -
Description
Rabbit polyclonal to IKZF4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide within Mouse IKZF4 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q8C208 -
Positive control
- This antibody gave a positive signal in both Mouse and Rat Brain tissue lysates.
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General notes
This product was previously labelled as ZNFN1A4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 64 kDa).Target
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Function
DNA-binding protein that binds to the 5'GGGAATRCC-3' Ikaros-binding sequence. Transcriptional repressor. Interacts with SPI1 and MITF to repress transcription of the CTSK and ACP5 promoters via recruitment of corepressors SIN3A and CTBP2. May be involved in the development of central and peripheral nervous systems. -
Tissue specificity
Highly expressed in skeletal muscle, low levels of expression in heart, thymus, kidney, liver, and spleen. Expressed in the hematopoietic cell lines MOLT-4, NALM-6 and K562. Highly expressed in THP-1 and Mo7e-IL3 cell lines, which have characteristics of myeloid and early megakaryocytic cells respectively. -
Sequence similarities
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Domain
The N-terminal zinc fingers are involved in sequence-specific DNA binding and heterotypic associations with other family members.
C-terminal zinc fingers mediate homodimerization. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 22781 Mouse
- Entrez Gene: 100361923 Rat
- SwissProt: Q8C208 Mouse
- Unigene: 358648 Mouse
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Alternative names
- EOS antibody
- Eos protein antibody
- IKAROS family zinc finger 4 (Eos) antibody
see all
Images
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Western blot - Anti-IKZF4 antibody (ab155754)All lanes : Anti-IKZF4 antibody (ab155754) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Exposure time: 16 minutesPlease note that Mouse Liver tissue lysate in lane 3 represents a negative control for IKZF4.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155754 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Datasheets and documents
References (0)
ab155754 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-IKZF4 antibody (ab155754)All lanes : Anti-IKZF4 antibody (ab155754) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 200 kDa (possible non-specific binding), 42 kDa (possible non-specific binding)
Exposure time: 16 minutesPlease note that Mouse Liver tissue lysate in lane 3 represents a negative control for IKZF4.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab155754 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
