Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human

Overview

  • Product name

    Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free
    See all IKZF3 Degron tag primary antibodies

  • Description

    Mouse monoclonal [1C3] to IKZF3 Degron tag - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details
    Unsuitable for: ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate. ELISA: Human IKZF3 Degron tag.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269478 is the carrier-free version of ab269464. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab269478 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    1C3

  • Isotype

    IgG1

Images

  • Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
    Western blot - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
    All lanes : Anti-IKZF3 Degron tag antibody [1C3] (ab269464) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control), containing a myc-mCherry, whole cell lysate
    Lane 2 : HEK-293T transfected with human IKZF3 Degron containing a myc-mCherry, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Exposure time: 10 seconds


    This data was produced using ab269464, the same antibody clone but in a different formulation.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
    ELISA - Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

    This data was produced using ab269464, the same antibody clone but in a different formulation.

    ab269464 used at 0-4000 ng/ml.

    Antigen (Human IKZF3 Degron tag) used at 1000 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.

  • Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)
    Anti-IKZF3 Degron tag antibody [1C3] - BSA and Azide free (ab269478)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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