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Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [R10Z8E9] to IgG - BSA and Azide free
  • Suitable for: ELISA, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IgG antibody [R10Z8E9] - BSA and Azide free
    See all IgG primary antibodies

  • Description

    Mouse monoclonal [R10Z8E9] to IgG - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • ELISA: Human IgG1. WB: Human serum lysate.

  • General notes

    ab256120 is the carrier-free version of ab124055. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    R10Z8E9

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
    Western blot - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
    Anti-IgG antibody [R10Z8E9] (ab124055) at 1/1000 dilution + Human serum lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366)

    Predicted band size: 36 kDa


    Exposure time: 3 minutes


    This image was produced using ab124055, the same clone but in a different formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Expected MW: 50 kDa.

  • ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
    ELISA - Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)

    This image was produced using ab124055, the same clone but in a different formulation.

    50ng Human IgG1 (50μL at 1000ng/mL) was coated onto 96-wells. Serial dilutions (0, 0.001, 0.005, 0.020, 0.078, 0.313, 1.25, 5μg/mL) of ab124055 (50μL) were incubated with Human IgG1 for 60 minutes by shaking. This was followed by adding goat anti-mouse IgG, (H+L), phosphatase-conjugated secondary antibody 1/1000 diluted (50μL) into each well and incubating for another 40 minutes. After washing, 50 μL of PNPP was added and incubated for 13 minutes without shaking for color development. OD was read at 405nm within 5 minutes. 

  • Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)
    Anti-IgG antibody [R10Z8E9] - BSA and Azide free (ab256120)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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