Antibodies, Proteins, Kits and Reagents for Life Science

261 331 ₸ Product size

100 µl 261 331 ₸

Order now and get it on Thursday March 04, 2021

Key features and details

Mouse monoclonal [OTI3G8] to IFIT1
Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human, African green monkey
Isotype: IgG1

Product name

Anti-IFIT1 antibody [OTI3G8]
See all IFIT1 primary antibodies
Description

Mouse monoclonal [OTI3G8] to IFIT1
Host species

Mouse

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human African green monkey
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
Database link: P09914
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow: HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
General notes

The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. The product can be stored for up to 12 months.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA
Concentration information loading...
Purity

IgG fraction
Purification notes

ab118062 is purified from TCS by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI3G8
Isotype

IgG1
Research areas

Western blot - Anti-IFIT1 antibody [OTI3G8] (ab118062)

All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution

Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 55 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
Immunocytochemistry/ Immunofluorescence - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
Flow Cytometry - Anti-IFIT1 antibody [OTI3G8] (ab118062)

ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry.

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