Anti-IFIT1 antibody [OTI3G8] (ab118062)
Key features and details
- Mouse monoclonal [OTI3G8] to IFIT1
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-IFIT1 antibody [OTI3G8]
See all IFIT1 primary antibodies -
Description
Mouse monoclonal [OTI3G8] to IFIT1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanAfrican green monkeyIHC-P HumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human IFIT1. Recombinant full length Human IFIT1 produced in HEK293T cells (NP_000899).
Database link: P09914 -
Positive control
- WB: HEK293T cell lysate transfected with pCMV6-ENTRY IFIT1 cDNA. IHC-P: Human liver carcinoma, Human ovary adenocarcinoma, kidney and liver tissues. ICC/IF: HT29 cells and COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 cDNA. Flow: HEK293T cells transfected with pCMV6-ENTRY IFIT1 overexpress plasmid
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General notes
The clone number has been updated from 3G8 to OTI3G8, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. The product can be stored for up to 12 months. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 48% PBS, 1% BSA -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
ab118062 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3G8 -
Isotype
IgG1 -
Research areas
Images
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All lanes : Anti-IFIT1 antibody [OTI3G8] (ab118062) at 1/500 dilution
Lane 1 : HEK293T cell lyaste, transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate, transfected with pCMV6-ENTRY IFIT1 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC203320) -
ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Liver carcinoma tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in Paraffin-embedded Human Ovary adenocarcinoma tissue by Immunohistochemistry.
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ab118062 at 1/50 dilution staining IFIT1 in COS7 cells transiently transfected by pCMV6-ENTRY IFIT1 by Immunofluorescence.
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ab118062 at 1/50 dilution staining IFIT1 in HT29 cells by Immunofluorescence.
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ab118062 at 1/100 dilution staining IFIT1 in HEK293T cells transfected with either pCMV6-ENTRY IFIT1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by Flow Cytometry.