Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)
![Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)](https://www.abcam.com/ps/products/251/ab251438/Images/ab251438-408277-anti-idi1-idi2-antibody-epr17213-bsa-and-azide-free-western-blot.jpg "Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17213] to IDI1+IDI2 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free
See all IDI1+IDI2 primary antibodies -
Description
Rabbit monoclonal [EPR17213] to IDI1+IDI2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251438 is the carrier-free version of ab205617. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251438 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR17213 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution
All lanes : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesThis data was developed using ab205617, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)](https://www.abcam.com/ps/products/251/ab251438/Images/ab251438-406048-anti-idi1-idi2-antibody-epr17213-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution + Human fetal liver tissue lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesThis data was developed using ab205617, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)](https://www.abcam.com/ps/products/251/ab251438/Images/ab251438-403399-anti-idi1-idi2-antibody-epr17213-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal skeletal muscle lysate
Lane 3 : Human colon cancer lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 3 minutesThis data was developed using ab205617, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)](https://www.abcam.com/ps/products/251/ab251438/Images/ab251438-399207-anti-idi1-idi2-antibody-epr17213-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)All lanes : Anti-IDI1 + IDI2 antibody [EPR17213] (ab205617) at 1/5000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 2 minutesThis data was developed using ab205617, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 2minutes; Lane 3: 3minutes.
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![Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)](https://www.abcam.com/ps/products/251/ab251438/Images/ab251438-5-benefits-of-recombinant-antibodies.png)
Anti-IDI1+IDI2 antibody [EPR17213] - BSA and Azide free (ab251438)