Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [KMab-1] to IDH2 (mutated R172K) - BSA and Azide free
  • Suitable for: WB, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free
    See all IDH2 primary antibodies

  • Description

    Rat monoclonal [KMab-1] to IDH2 (mutated R172K) - BSA and Azide free

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Western blot - Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)
    Western blot - Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)

    This data was produced using ab264052, the same clone in a different formulation.

    ab264052 used at 1/1000 dilution (0.805 μg/ml).

    Secondary used: Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10,000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The loading samples are E.coil extracts containing recombinant protein respectively.

    Lane 1:  His-tagged human IDH1 (aa1-414) recombinant protein 10ng

    Lane 2:  His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng

    Lane 3:  His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng

    Lane 4:  His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng

    Lane 5:  His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng

    Lane 6:  His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng

    Lane 7:  His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng

    Lane 8:  His-tagged human IDH2 (aa40-452) recombinant protein 10ng

    Lane 9:  His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng

    Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng

    Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng

    Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng

    Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng

    Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng

  • ELISA - Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)
    ELISA - Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)

    This data was produced using ab264052, the same clone in a different formulation.

    ab264052 used at 0-5000 ng/ml.

    Antigen (IDH2, IDH2(R172G), IDH2(R172M), IDH2(R172S), IDH2(R172W), IDH2(R172K), IDH2(R140Q)) used at 100 ng/ml.

    Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.

  • Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)
    Anti-IDH2 (mutated R172K) antibody [KMab-1] - BSA and Azide free (ab264060)

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