Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
![Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)](https://www.abcam.com/ps/products/272/ab272697/Images/ab272697-2-anti-icos-ligandicosl-antibody-epr23289152-flow-cytometry-huvec.jpg "Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free
See all ICOS Ligand/ICOSL primary antibodies -
Description
Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Jurkat, HUVEC cells.
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General notes
ab272697 is the carrier-free version of ab233554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23289-152 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) cells treated with 200U/ml TNF alpha for 24h (Red) / Untreated control (Green) cells labelling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells (PMID: 16210590).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).
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![Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)](https://www.abcam.com/ps/products/272/ab272697/Images/ab272697-1-anti-icos-ligandicosl-antibody-epr23289152-flow-cytometry-jurkat.jpg)
Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labeling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 21851236). Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).
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![Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)](https://www.abcam.com/ps/products/272/ab272697/Images/ab272697-3-benefits-of-recombinant-antibodies.png)
Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
