Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free
  • Suitable for: Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free
    See all ICOS Ligand/ICOSL primary antibodies

  • Description

    Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: Jurkat, HUVEC cells.

  • General notes

    ab272697 is the carrier-free version of ab233554. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23289-152

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
    Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

    Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) cells treated with 200U/ml TNF alpha for 24h (Red) / Untreated control (Green) cells labelling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells (PMID: 16210590).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).

  • Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
    Flow Cytometry - Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

    Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji (Human Burkitt's lymphoma B lymphocyte, Right) cells labeling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 21851236). Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab233554).

  • Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)
    Anti-ICOS Ligand/ICOSL antibody [EPR23289-152] - BSA and Azide free (ab272697)

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