Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20718] to ICA 512/PTPRN
  • Suitable for: WB, IHC-Fr, IP, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ICA 512/PTPRN antibody [EPR20718]
    See all ICA 512/PTPRN primary antibodies

  • Description

    Rabbit monoclonal [EPR20718] to ICA 512/PTPRN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    Human
    IP
    Mouse
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human hippocampus and pancreas tissue lysate; mouse brain, hippocampus and hypothalamus tissue lysate; rat brain, hippocampus and pancreas tissue lysate. IP: Mouse hypothalamus tissue lysate. IHC-P: Human pancreas tissue; mouse adrenal gland tissue; rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; rat pancreas tissue.

  • General notes

    Previously labelled as PTPIA2 / PTPRN. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20718

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/5000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Mouse hypothalamus lysate
    Lane 3 : Mouse brain lysate
    Lane 4 : Mouse hippocampus lysate
    Lane 5 : Rat hippocampus lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 106 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545; PMID: 18824546).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive cytoplasmic staining in mouse pancreatic islet cells is observed (PMID: 10820150). 

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

    Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunoprecipitation - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    ICA 512/PTPRN was immunoprecipitated from 0.35 mg of mouse hypothalamus tissue lysate with ab207750 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207750 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: Mouse hypothalamus lysate 10 μg (Input).
    Lane 2: ab207750 IP in mouse hypothalamus tissue lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207750 in mouse hypothalamus tissue lysate (-).
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time:

  • Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/1000 dilution

    Lane 1 : Rat pancreas lysate
    Lane 2 : Human pancreas lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 106 kDa
    Observed band size: 65 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST. 

    Exposure times.

    Lane 1: 3 minutes

    Lane 2: 5 seconds

    The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545; PMID: 18824546).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on chromaffin cells of mouse adrenal medulla is observed (PMID: 8641276). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Weak positive cytoplasmic staining in rat pancreatic islet cells is observed (PMID: 10820150).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/500 dilution.

    Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)
    Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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