Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20718] to ICA 512/PTPRN
Suitable for: WB, IHC-Fr, IP, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-ICA 512/PTPRN antibody [EPR20718]
See all ICA 512/PTPRN primary antibodies
Description

Rabbit monoclonal [EPR20718] to ICA 512/PTPRN
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat Human
IP	Mouse
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human hippocampus and pancreas tissue lysate; mouse brain, hippocampus and hypothalamus tissue lysate; rat brain, hippocampus and pancreas tissue lysate. IP: Mouse hypothalamus tissue lysate. IHC-P: Human pancreas tissue; mouse adrenal gland tissue; rat pancreas tissue. IHC-Fr: Mouse pancreas tissue; rat pancreas tissue.
General notes
Previously labelled as PTPIA2 / PTPRN.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20718
Isotype

IgG
Research areas

Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/5000 dilution

Lane 1 : Human hippocampus lysate
Lane 2 : Mouse hypothalamus lysate
Lane 3 : Mouse brain lysate
Lane 4 : Mouse hippocampus lysate
Lane 5 : Rat hippocampus lysate
Lane 6 : Rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

Developed using the ECL technique.

Predicted band size: 106 kDa
Observed band size: 65 kDa
why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545; PMID: 18824546).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on human pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized mouse pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive cytoplasmic staining in mouse pancreatic islet cells is observed (PMID: 10820150).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunoprecipitation - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

ICA 512/PTPRN was immunoprecipitated from 0.35 mg of mouse hypothalamus tissue lysate with ab207750 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab207750 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse hypothalamus lysate 10 μg (Input).
Lane 2: ab207750 IP in mouse hypothalamus tissue lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207750 in mouse hypothalamus tissue lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time:
Western blot - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

All lanes : Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750) at 1/1000 dilution

Lane 1 : Rat pancreas lysate
Lane 2 : Human pancreas lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 106 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times.

Lane 1: 3 minutes

Lane 2: 5 seconds

The molecular weight observed is consistent with what has been described in the literature (PMID: 15596545; PMID: 18824546).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on chromaffin cells of mouse adrenal medulla is observed (PMID: 8641276). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat pancreatic islets is observed (PMID: 8641276). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Frozen sections) - Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

Immunohistochemical analysis of 4% PFA-fixed, 0.2 % Triton X-100 permeabilized rat pancreas tissue labeling ICA 512/PTPRN with ab207750 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Weak positive cytoplasmic staining in rat pancreatic islet cells is observed (PMID: 10820150).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/500 dilution.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
Anti-ICA 512/PTPRN antibody [EPR20718] (ab207750)

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