Anti-I-FABP antibody (ab60272)
Key features and details
- Goat polyclonal to I-FABP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-I-FABP antibody
See all I-FABP primary antibodies -
Description
Goat polyclonal to I-FABP -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Human I-FABP aa 121-132 (C terminal).
Sequence:C-EGVEAKRIFKKD
(Peptide available asab115238) -
Positive control
- WB: Human Duodenum lysate. IHC-P: Human small intestine.
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General notes
Previously labelled as intestinal FABP.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-I-FABP antibody (ab60272)ab60272 antibody (0.001µg/ml) staining of I-FABP in Human Duodenum lysate (35µg protein in RIPA buffer)
Predicted band size: 15 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-I-FABP antibody (ab60272)ab60272 staining I-FABP in Human small intestine by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The tissue sections underwent antigen retrieval by steam with citrate buffer pH 6.0. The AP staining procedure was used for detection.
