Anti-HuR / ELAVL1 antibody (ab238528)
Key features and details
- Rabbit polyclonal to HuR / ELAVL1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HuR / ELAVL1 antibody
See all HuR / ELAVL1 primary antibodies -
Description
Rabbit polyclonal to HuR / ELAVL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human HuR/ ELAVL1 aa 1-350. NP_001410.2
Database link: Q15717 -
Positive control
- WB: U-251 MG, HeLa, Jurkat, K562 and PC-3 cell extracts. IHC-P: Rat brain and testis tissue. Mouse testis and kidney tissue; Human colon, esophagus, esophageal cancer, stomach, gastric cancer, kidney and kidney cancer tissue; Rat cancer tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-HuR / ELAVL1 antibody (ab238528)All lanes : Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution
Lane 1 : Wild-type HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : HuR / ELAVL1 knockout HEK-293 cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 36 kDa
Exposure time: 90 secondsBlocking buffer: 3% nonfat dry milk in TBST.
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Western blot - Anti-HuR / ELAVL1 antibody (ab238528)All lanes : Anti-HuR / ELAVL1 antibody (ab238528) at 1/1000 dilution
Lane 1 : U-251 MG (Human brain glioma cell line) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts
Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell extracts
Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) cell extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 36 kDaBlocking buffer: 3% non-fat dry milk in TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human colon tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded rat testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded mouse testis tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded rat brain tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded mouse kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human esophagus tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human esophageal cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human stomach tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human gastric cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human kidney tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded human kidney cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuR / ELAVL1 antibody (ab238528)Paraffin-embedded rat cancer tissue stained for HuR / ELAVL1 with ab238528 at a 1/200 dilution in immunohistochemical analysis.
