Anti-Human IgM antibody [RM121] (ab193159)
![Anti-Human IgM antibody [RM121] (ab193159)](https://www.abcam.com/ps/products/193/ab193159/Images/ab193159-230573-anti-human-igm-antibody-rm121-elisa.jpg "Anti-Human IgM antibody [RM121] (ab193159)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM121] to Human IgM
- Suitable for: Sandwich ELISA, ELISA
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Human IgM antibody [RM121]
See all Human IgM primary antibodies -
Description
Rabbit monoclonal [RM121] to Human IgM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragment
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Immunogen
Full length protein corresponding to Human Human IgM. Human IgM
Database link: P01871
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM121 -
Isotype
IgG -
Research areas
Images
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ELISA - Anti-Human IgM antibody [RM121] (ab193159)
A titer ELISA using ab193159. The plate was coated with different amounts of human IgM. A serial dilution of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)](https://www.abcam.com/ps/products/193/ab193159/Images/ab193159-230570-anti-human-igm-antibody-rm121-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![ELISA - Anti-Human IgM antibody [RM121] (ab193159)](https://www.abcam.com/ps/products/193/ab193159/Images/ab193159-230572-anti-human-igm-antibody-rm121-elisa.jpg)
ELISA - Anti-Human IgM antibody [RM121] (ab193159)ELISA of human immunoglobulins shows ab193159 only reacted to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of ab193159 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)](https://www.abcam.com/ps/products/193/ab193159/Images/ab193159-230571-anti-human-igm-antibody-rm121-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgM antibody [RM121] (ab193159)Sandwich ELISA using ab193159 as the capture antibody (100ng/well), and a biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
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![Anti-Human IgM antibody [RM121] (ab193159)](https://www.abcam.com/ps/products/193/ab193159/Images/ab193159-5-benefits-of-recombinant-antibodies.png)
Anti-Human IgM antibody [RM121] (ab193159)
