Anti-HSD17B1 antibody [EP1682Y] (ab51045)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1682Y] to HSD17B1
- Suitable for: WB, Flow Cyt, IP, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-HSD17B1 antibody [EP1682Y]
See all HSD17B1 primary antibodies -
Description
Rabbit monoclonal [EP1682Y] to HSD17B1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human HSD17B1 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Human placenta lysate. IHC-P: Human placenta tissue. IP: Human placenta lysate.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1682Y -
Isotype
IgG -
Research areas
Images
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Anti-HSD17B1 antibody [EP1682Y] (ab51045) at 1/100000 dilution + Human placenta lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
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Ab51045 (1/100 dilution) staining human HSD17B1 in paraffin embedded human placenta tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Purified ab51045 at 1/50 dilution (2µg) immunoprecipitating HSD17B1 in Human placenta lysate.
Lane 1 (input): Human placenta lysate 10µg
Lane 2 (+): ab51045 + Human placenta lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51045 in Human placenta lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 34 kDa -
Overlay histogram showing JEG3 cells stained with ab51045 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51045, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
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