Anti-HPV18 E7 antibody [8E2] (ab100953)
![Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114068-anti-hpv18-e7-antibody-8e2-western-blot.jpg "Anti-HPV18 E7 antibody [8E2] (ab100953)")
Key features and details
- Mouse monoclonal [8E2] to HPV18 E7
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG1
Overview
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Product name
Anti-HPV18 E7 antibody [8E2] -
Description
Mouse monoclonal [8E2] to HPV18 E7 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Human papillomavirus -
Immunogen
Recombinant full length HPV18 E7 protein
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Epitope
Mapped to amino acids 36-70 -
Positive control
- WB: U20S, Hela and SiHa cell lysates. ICC/IF: U2OS cells transfected with plasmid expressing HPV18 E7 protein; Hela cells.
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General notes
Monoclonal antibody working titer has to be established practically for each particular antigen and assay format.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
8E2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-HPV18 E7 antibody [8E2] (ab100953)All lanes : Anti-HPV18 E7 antibody [8E2] (ab100953) at 1/1000 dilution
Lane 1 : lysate of U20S cells with plasmid expressing E7 protein
Lane 2 : plain U20S lysate
Lane 3 : Hela lysate. HeLa cells containing HPV-18 genome sequences expressing HPV E6 and E7 proteins.
Lane 4 : SiHa lysate
Lane 5 : Marker
Predicted band size: 12 kDa
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![Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114067-anti-hpv18-e7-antibody-8e2-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HPV18 E7 antibody [8E2] (ab100953)IP analysis of HPV18 E7 protein using ab100953 at 1/500:
Lane 1: detection of E7 protein in lysate of U20S cells
Lane 2: the lower band is precipitated E7, upper bands are light and heavy chain of antibody
Lane 3: precipitation without ab100953
Lane 4: IP exp. with plain U2OS cells
Lane 5: IP exp. with non-transfected HeLa cells. Lower band corresponds to native HPV-18 E7 protein expressed by HPV-18 genome expressed in Hela cells.
Lane 6: marker. -
![Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)](https://www.abcam.com/ps/products/100/ab100953/Images/ab100953-114066-anti-hpv18-e7-antibody-8e2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HPV18 E7 antibody [8E2] (ab100953)Immunofluorescent (IF) analysis of HPV18 E7 protein in U2OS cells. IF was carried out with U2OS cells transfected with plasmid expressing HPV18 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV18 E7 detected by ab100953. IF analysis of E7 protein shows similar result also in HeLa cells.